Abstract
In acute toxicity test of riboflavin-tetranicotinate, mice and rats were administered orally with 1.0 g/kg body weight and 5.0 g/kg body weight of this compound, respectively, but no death was found after 72 hours of administration. No difference was found in appearance and in appetite, between animals tested and those of control. Chronic toxicity of this compound was examined using male albino rats of Wistar strain as test animals. They were administered compulsorily with 230 mg/day/kg body weight of this compound. The body weight gains of the rats tested were the same with those of control rats. After 6 months' feeding, the measurement of weight of each organ, hematological examination on blood, and histological investigation on organs and tissues were performed. No difference was found between rats fed on riboflavin-tetranicotinate and those of control, indicating that this compound has no chronic toxicity up to the dose of 230 mg/day/kg.
