THE EFFECT OF CRUDE TOXOHORMONE ON THE METABOLISM OF PANTOTHENIC ACID

Abstract

The total and free pantothenic acid levels of the tissues were determined in mouse hepatoma MH-134,Sarcoma-180 (solid and ascites types) and normal mouse liver. Low values (12.5% of normal level) of total pantothenic acid were shown in hepatoma MH-134,while both types of Sarcoma-180 were almost same to the value of hepatoma MH-134. On the other hand, high percentages of free pantothenic acid were noted in hepatoma MH-134 and solid type of Sarcoma-180. It seemed that the reason for this high percentage was due to containing considerable amount of dead cells in their tissues. The values of coenzyme A in livers which prepared from the mice bearing Sarcoma-180 ascites tumor were found to be lower in comparison with that of the normal. The injection of the crude toxohormone, prepared from Sarcoma-180 by Nakahara's method, resulted in decline of coenzyme A level of the normal liver. These facts may suggest the action of toxohormone on pantothenic acid metabolism.