MICROAUTORADIOGRAM OF THIAMINE : (I) UPTAKE OF THIAMINE HYDROCHLORIDE-^3H AND O-BENZOYL-THIAMINE DISULFIDE-^3H IN GENERAL VISCERAE OF THE MICE WITH PERORAL ADMINISTRATION

Abstract

Uptake of thiamine hydrochloride-^3H (B_1-^3H) and O-benzoylthiamine disulfide-^3H in various organs of the mice with oral administration has been studied by microautoradiogram. The tissue obtained from the experimental animals were fixed in cold absolute alcohol at -2O℃. Paraffin sections were made and dipping method was employed for autoradiographic technique. Both B_1-^3H and BTDS-^3H were mostly absorbed from the upper half of the small intestine, though a small amount of BTDS-^3H was absorbed from the stomach. BTDS-^3H passed out the absorption epithelium more quickly than B_1-^3H. The liver, heart, kidneys, pancreas, adrenals, hypophysis, testes, muscle, thyroid gland, spleen and lungs, etc. revealed marked the uptake of both B_1-^3H and BTDS-^3H. But the uptake of BTDS-^3H in these organs was more marked than it of B_1-^3H. Distribution pictures of grains in general viscerae were described and demonstrated in 40 pictures. Throughout this observation, following findings were pointed out. (1) In the cells the grains were found in cytoplasm, nucleus and nucleolus. (2) Marked uptake of B_1-^3H and BTDS-^3H was found in β-cells of Langerhans islets of the pancreas, cortical cells, especially in zona fasciculata of the adrenals, spermatic cells in the testes, follicular epithelia in thyroid gland besides in liver cell, muscle cells of the heart, and urinary epithelium of the kidneys, etc.