Abstract
Kloeckera apiculata cells repeatedly centrifuged at 1,500 rpm, were incubated in the broth at higher concentration of cell-counts for 2 or 4 hours and then they wer diluted with the broth containing thiamine or its derivatives ; synchronized growth of cells was observed on incubation for 2-6 hours. When thiamine, thiamine propyldisulfide (TPD) or S-carbethoxy-thiamine (CET) was added to the broth (0.125 μg equivalent), TPD stimulated the growth more quickly than thiamine at the first stage of growth, whereas CET and thiamine showed parallel synchronized growth, until at the last stage of logarithmic growth CET stimulated one more synchronized growth than thiamine. These differences of synchronized growth by TPD and CET will be originated from their different speed of conversion to thiamine.
