Abstract
A new method of the separation and determination of thiamine and its phosphoric esters (TMP and TDP) is described. By carboxylic acid ion exchanger (Amberlite IRC-50), thiamine and TMP were selectively taken up from the mixture of thiamine, TMP, and TDP, on the other hand TDP was not adsorbed by the resin but passed down completely. Thiamine and TMP can be removed from the resin with 0.05N HCl. Thiamine and TMP were adsorbed to activated zeolite (Permutit) and thiamine was selectively eluted with 25% KCl in 0.1N HCl, but TMP was not. Finally TMP, which was adsorbed to the zeolite, was eluted with 2N HCl completely. Each of these fractions (thiamine, TMP and TDP) is available to determination by thiochrome method.
