Abstract
The study was made on the separation and the determination of thiamine and its phosphates in animal tissues. It was confirmed that thiamine and its phosphates could be separated completely with success by means of a stepwise DEAE・Sephadex A-25 column chromatography in which elution was made with 0.1,0.2 and 0.3M formic acid solution. Thiamine is determined by thiochrome method. It was demonstrated that this procedure is rather simple, very ready to perform and the recovery rate of thiamine and its phosphates added to the column was about 100 per cent.
