Abstract
Rose's hemolysis test using dialuric acid was simplified and the following facts were observed by the method. Dialuric acid, H_2O_2 and peroxidized ethyl linoleate specifically hemolyzed the vitamin E-deficient erythrocytes under certain conditions, although HgCl_2,KMnO_4 and Tween 80 equally hemolyzed the erythrocytes of vitamin E-deficient and normal rats. Vitamin K_3,K_2CrO_4 and K_2Cr_2O_7 had the antihemolytic effect on in vitro dialuric acid-induced hemolysis of vitamin E-deficient erythrocytes. The method was expected to be used as a simple biological assay procedure of vitamin E.
