STUDIES ON THE DETERMINATION OF VITAMIN A IN THE PRESENCE OF VITAMIN E : (II) DETERMINATION OF VITAMINS A AND E IN THEIR MIXED PREPARATIONS BY HYDROGENATION METHOD

Abstract

The determination of vitamins A and E in their mixed preparations was examined. Hydrolysis of the samples was carried out in ethanolic potassium hydroxide by addition of antioxidants such as hydroquinone, pyrogallol or their mixtures. The unsaponifiable fraction was extracted with ether in usual method. The extracted vitamins A and E were assayed by hydrogenation method described in previous paper. The results obtained using the proposed procedure on a variety of preparations containing vitamins A and E were almost quantitative.