Abstract
Formation of desthiothiamine (IV) and the diazepine compound (V) from thiamine in its alkaline solution was demonstrated by means of paper partition chromatography. Thiamine-HCl with equimolar glycine in a trimolar NaOH solution at 30℃ for 48 hours evolved the highest amount of H_2S than in an equi-, di- or tetramolar NaOH solution, but at the absence of glycine evolution of H_2S was greatly decreased. When the above desulfurization of thiamine with or without glycine was carried out at 100℃ for 3 hours, the amount of evolved H_2S was remarkably increased. The presence of glycine increased formation of desthiothiamine, but decreased extremely formation of the diazepine compound. Desthiothiamine was obtained from the diazepine compound by heating its acetate buffer solution (pH5.0) on boiling water bath for 10 hours. This conversion was not effected by addition of glycine. Thiamine was also fomed from the diazepine compound by saturation of H_2S in its acetate buffer solution (pH5.0) at 90℃.
