Abstract
At first, analytical investigations were performed for the separation and identification of 3-[2'-methyl-4'-aminopyrimidyl-(5')]-methyl-4-methyl-5-β-chloroethylthiazolium (chlorothiamine). The chlorothiamine was possibly estimated by the application of the paper chromatography, followed by the ordinary thiochrome method. In case of pure chlorothiamine added to poultry urine or feces, the method was found to be available for the desired recovery of the compound, yielding 98〜102% in feces, 98〜103% in urine. Then, the metabolic fate of chlorothiamine was investigated by poults fitted an artificial anus with cannula. When ^<35>S-chlorothiamine was orally administered, urinary excretion of ordinary thiamine was continued for 24 hours, while that of chlorothiamine for 12 hours. The recovery of ^<35>S (cpm) was seen to be 82% in urine and 18% in feces, whereas the recoveries of ^<35>S-chlorothiamine were 24% in urine and 5.4% in feces. From the data, it could not be realized that chlorothiamine may be convertible to the ordinary thiamine in vivo.
