Abstract
Possibility and efficiency of Amberlite XAD-1 and XAD-2,new adsorbents of synthetic insoluble crosslinked polystylene polymer, were examined for purification of corrinoids using DBC, FB and DBCC. XAD-2 was superior to XAD-1 in the ability of adsorbing DBC. Further examination showed that XAD-2 is an excellent adsorbent for purification of corrinoids, so far as the compounds tested are concerned. As eluants for corrinoids from XAD-2,several kinds as well as several concentrations of alcohols and acetone were tested. For elution of DBC as well as DBCC, satisfactory results were obtained with methanol and acetone of the concentrations more than 60% and 50%, respectively. For FB, addition of KCN improved its elution with the both solvents. The applicability of XAD-resins to the purification of corrinoids in natural substances was also confirmed using the corrinoid extracts from Propionibacterium shermanii and Pr. arabinosum.
