ON THE DETERMINATION OF THIAMINE IN THE MODIFIED MILK POWDER : (II)APPLICATION OF THE PRETRETED METHOD BY PROTEASE TO COMMERCIAL MODIFIED MILK POWDER PREPARATION
1969 Volume 39 Issue 2 Pages 109-112
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1969 Volume 39 Issue 2 Pages 109-112
The authors have found that the thiamine value in a modified milk powder "Lebensmilk A", by ordinary hydrochloric extraction method gave a poor recovery. Therefore, it was improved the procedure by a preliminary treatment with protease of Streptomyces griceus and Takadiastase, which was able to increase the recovery rate to 120% against the ordinary method. In this paper, the results of determination of thiamine value by a improved method in 7 commercial milk powder preparations are described. The recovery rates of added thiamine were shown to be 109-130%, average ll7% for [ordinary procedu e. The improved method has also better reproducibility. It is supposed to be suitable for the practical procedure for the determination of thiamine in commercial preparations of modified milk powder.
