VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
Volume 39, Issue 2
Displaying 1-22 of 22 articles from this issue
  • Kenji YAMAGA
    Article type: Article
    1969 Volume 39 Issue 2 Pages 77-85
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    The effect of Pronase, an enzyme preparation of Streptomyces, on vitamin B_<12> absorption from rat intestinal loops was studied in comparison with that of intrinsic factor. It has been found that Pronase does not bind B_<12> in vitro as does intrinsic factor, and that its effect is quite different from that of intrinsic factor. Absorption of B_<12> under the influence of Pronase was much slower than that effected by intrinsic factor, and Pronase had no effects on the absorption of radioactive iodide, chromate or thiamine (-^<35>S), suggesting that Pronase does not increase permeability of the mucosa but specifically enhances absorption of B_<12>. It has also been found that in the loop, B_<12> is converted within one hour to a state where intrinsic factor can no longer act on B_<12>, but Pronase does increase absorption of such B_<12>, and that Pronase does not destroy the so-called"mucosal receptor."
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  • Ichiro KUROKAWA, Toshiyuki KIMURA, Tomoko KOBAYASHI, Eiko OHASHI, Yosh ...
    Article type: Article
    1969 Volume 39 Issue 2 Pages 86-90
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    The influence of α-tocopherylacetate on the counts of erythrocytes, reticulocytes, thrombocytes, leucocytes, hemoglobin and hematocrit value and blood picture in the venous blood (EDTA-K_3 as anticoagulant) were studied for 24 hours after sampling. The blood were obtained from 5 normal persons, and α-tocopherylacetate was added with the concentration of 50mg%, and the following results were obtained. The erythrocytes, reticulocytes, hemoglobin and hematocrit values kept constant both in control and E group^*. The decrease of thrombocytes was seemingly more marked in control group and the morphological stabilities were fairly good in E group. The decrease of leucocytes counts were good controlled in E group for 24 hours. The gradual increase of lymphocytes and the decrease of segmented neutrophile leucocytes were evidently marked in control group, while in E group both lymphocytes and neutrophile leucocytes were fairly constant within 6 hours.
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  • Masashi YAMAGUCHI
    Article type: Article
    1969 Volume 39 Issue 2 Pages 91-96
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    It has been reported by several authors that the riboflavin level of tumor-bearing animal is lower than that of normal one. The present author determined the total and three types of riboflavin in liver of tumor-bearing rat. The decrease of total riboflavin to 2/3 of normal was recognized on 3-4 weeks after the plantation of tumor. A marked decrease to 1/2 of normal was found in mitochondrial fraction of liver, while in microsomal fraction it showed almost constant level through the experimental period.
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  • Masashi YAMAGUCHI
    Article type: Article
    1969 Volume 39 Issue 2 Pages 97-103
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    It is well known that liver catalase activity of tumor-bearing animals is significantly lower than that of normal animals. Nakahara, et al. suggested that certain toxic substances might be produced in tumor tissue which caused decrease of liver catalase activity of tumor-bearing animal, and it was called "toxohormone." We reported before that the riboflavin content of tumor-bearing rat liver decreased significantly after the transplantation of tumor. In this report, the author studied the effect of toxohormone on rats liver riboflavin. It was clearly shown that toxohormone cause a decrease of riboflavin, especially in mitochondrial fraction. It was also discussed the effect of toxohormone on flavokinase and FAD-pyrophos-phorylase.
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  • Koki HIRAGA, Tadashi KAMIKUBO
    Article type: Article
    1969 Volume 39 Issue 2 Pages 104-108
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    The role of δ-aminolevulinic acid was studied in the biosynthesis of corrinoids and porphyrins with the resting cells of Propionibacterium shermanii. Anaerobic incubation resulted in the increased production of corrinoids and porphyrins, but majority of the former was incomplete (nucleotide-free) corrinoid carboxylic acids. It was also found that the use of the increased amount of δ-aminolevulinic acid in the anaerobic incubation resulted in the formation of more incomplete corrinoid poly(probably tetra and penta)-carboxylic acids. It is interesting that the aerobic incubation subsequent to the anaerobic permitted easily the conversion of incomplete corrinoids to the complete forms and the amidation of carboxylic side chains of corrin ring.
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  • Takeo ITO, Ritsuko SHIBASAKI, Kenji ARASE
    Article type: Article
    1969 Volume 39 Issue 2 Pages 109-112
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    The authors have found that the thiamine value in a modified milk powder "Lebensmilk A", by ordinary hydrochloric extraction method gave a poor recovery. Therefore, it was improved the procedure by a preliminary treatment with protease of Streptomyces griceus and Takadiastase, which was able to increase the recovery rate to 120% against the ordinary method. In this paper, the results of determination of thiamine value by a improved method in 7 commercial milk powder preparations are described. The recovery rates of added thiamine were shown to be 109-130%, average ll7% for [ordinary procedu e. The improved method has also better reproducibility. It is supposed to be suitable for the practical procedure for the determination of thiamine in commercial preparations of modified milk powder.
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  • Yoshitomo KOCHI, Keizo NAKAMURA
    Article type: Article
    1969 Volume 39 Issue 2 Pages 113-118
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    A color reaction of AsA^<*2)> viz DAsA^<*1)> with pyrrole was examined about the specificity and the reaction mechanism. It was reported in the previous paper that this reaction was specific to tricarbonyl compounds. However, it was found that some diketo- compounds such as isatin, glyoxal developed a blue color. On the other hand, it was known that some five-membered heterocyclic compounds such as furan, thiophen and pyrrolidine revealed no blue color, when they were used as the substitute of pyrrole. Pyrrole having a substituents at 2-position or at 2,5-positions produced no blue material in the reaction, but 3,4-dimethylpyrrole or 2,2'-bipyrrole developed the color with DAsA. As the results, it was presumed that DAsA reacted with two moles of pyrrole or with one mole of 2,2'-bipyrrole to produce a blue dye, the chemical structure of which resembled to indophenine. The study is now going on and the result will be reported in the future.
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  • Kunihei INAZU, Masayoshi INOUE, Ryuichi YAMAMOTO
    Article type: Article
    1969 Volume 39 Issue 2 Pages 119-124
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    No other reports have been found on the transformation of S-alkoxycarbonylthiamines such as S-ethoxycarbonylthiamine(CET) or O, S-bis(ethoxycarbonyl)thiamine(DCET)in alkaline aqueous solution. However, these derivatives are known to be determined with "alkali-method" on the basis of their easy transformation into thiochrome-reaction-positive compounds on treatment with NaOH in aqueous solution. The present experiments were made to obtain informations on the degradation of CET and DCET, and of O-ethoxycarbonylthiamine (OCET) in alkaline aqueous solution of a pH ranging from 10 to 13 at 30℃. CET readily gave OCET as a result of translocation of ethoxycarbonyl group from S- to O-position, and OCET was hydrolyzed to thiamine (B_1). DCET was hydrolyzed similarly to OCET and B_1,but not so readily as did CET. When O-butoxycarbonyl-S-ethoxycarbonylthiamine (OCBSCET) was treated under the same conditions as was DCET, OCBSCET gave B_1 OCET, and O-butoxycarbonylthiamine (OCBT). OCET was produced in larger amounts than OCBT. This indicates. easier hydrolysis of O-ester linkage than the S-ester linkage of OCBSCET and, presumedly, of DCET. Each of the degradation rate constant-pH profiles of OCET, CET, and DCET in NaOH aqueous solution was a line having a slope of unity, where variation of ionic strength gave no influence on the rate at a fixed pH. When the pH value was adjusted with a glycine buffer, of a fixed ionic strength, instead of NaOH aqueous solution, an increase in concentration of glycine produced an increase in the degradation rate of DCET considerably at pH 10.0,slightly at pH 11.0,but not at pH 12.0,while the rate of CET was remained unchanged at these pH.
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  • Kiyoshi TSUKIDA, Miya YOKOTA
    Article type: Article
    1969 Volume 39 Issue 2 Pages 125-128
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    The position of the two sec-hydroxyls in neoxanthin was revealed by studying the rather complicated reaction mixtures formed from the following reactions : l) The CHCl_3・HCl reagent and neoxanthin in benzene solution. 2) The CHCl_3・HCl reagent and neoxanthin in chlroform solution. 3) Neochrome and LiAlH_4. 4) The tetraol ex neochrome and POCl_3. The diol A, one of the products derived from the above reaction 3 was identical with natural zeaxanthin in all respects, while isozeaxanthin could not be found throughout all four reactions. Experimental results including those obtained in the previous reports strongly support the structure proposed by Weedon et al. The significance of the M-80 peak which appears in the mass spectra of epoxycarotenoids and is missing in general carotenesand xanthophylls was discussed.
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  • Kiyokazu OOMAE
    Article type: Article
    1969 Volume 39 Issue 2 Pages 129-136
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    In order to elucidate the mechanism of the high blood contents of vitamin B_<12> and increased urinary excretion, of B_<12> in human leukemia, the study of B_<12> metabolism in rats following whole body ^<60>Co γ-irradiation was undertaken. It is a well known fact that peripheral leukocytes show a considerable decrease within 3 days following LD_<50> irradiation, but when B_<12> metabolism was examined in the rat during the same period, it was found that the B_<12> level in blood showed a temporary significant increase during the 3 to 24 hour period following irradiation. Urinary excretion of B_<12> in the rat, even in controls, is more than 30 times as that in the human body, the weight of the rat being converted to that of the human body, but when the rat was irradiated, a significant increase in urinary excretion of B_<12> was found within 48 hours. It was postulated by the author that the increase in B_<12> level in blood within 24 hours, and increase in excretion of B_<12> within 48 hours after irradiation are attributed to B_<12> release from leukocyte destruction.
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  • [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 137-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 137-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 137-138
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 138-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 138-139
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 139-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 139-140
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 140-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 140-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 140-141
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
    Download PDF (344K)
  • [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 141-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 39 Issue 2 Pages 141-
    Published: February 25, 1969
    Released on J-STAGE: February 17, 2018
    JOURNAL FREE ACCESS
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