Abstract
A method for the separation and determination of vitamin A and α-tocopherol in mixtures by gel filtration using the column of Sephadex LH-20 was examined. Hydrolysis of the samples was carried out in ethanolic potassium hydroxide by addition of antioxidants. The unsaponifiable fraction was extracted with ether in usual method. The extracted vitamin A and α-tocopherol were fractionated on Sephadex LH-20 column as the gel bed and chloroform as the eluting solvent. After treatment of gel filtration vitamin A and α-tocopherol were determined using the spectrophotometrric assay for vitamin A and Emmerie・Engel assay for α-tocopherol. Separation of vitamin A and α-tocopherol was achieved successfully by this method and recoveries were satisfactory.
