VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 40, Issue 2

Displaying 1-35 of 35 articles from this issue

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (I) GROWTH OF SACCHAROMYCES CEREVISIAE AND ITS ACCUMULATION OF THIAMINE

Chikataro KAWASAKI, Toshihiro IIO, Masaomi KONDO

Article type: Article
1969 Volume 40 Issue 2 Pages 59-63
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_59

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Thiamine was easily adsorbed by cells of Sacch. cerevisiae in Reader's medium, when cells had been harvested at the exponential phase, but it was poorly adsorbed by cells at the later stationary phase. When the cells at the stationary phase had been incubated for growth in a fresh medium, the cells harvested, were demonstrated to be restored to adsorb thiamine strongly. The extraordinary accumulation of thiamine in yeast cells was shown to be correlated to their status of harvesting rate of growth, number of budding cells and higher contents of nucleic acids in cells, as shown in Fig. 3 and Table 3. It was suggested that the thiamine accumulation in yeast cells depended upon the active physiological state of the cells prior to the cell division.

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (II) GROWTH OF SACCHAROMYCES CEREVISIAE AND ITS BIOSYNTHESIS OF THIAMINE FROM PYRIMIDINE AND THIAZOLE MOIETIES

Chikataro KAWASAKI, Toshihiro IIO, Masaomi KONDO

Article type: Article
1969 Volume 40 Issue 2 Pages 64-66
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_64

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Saccharomyces cerevisiae can synthesize thiamine from its pyrimidine and thiazole moieties but the rate of synthesis varied with cells, harvested in different media and reaction media for synthesis. The highest yield on synthesis was demonstrated by cells harvested in Atkin's medium and by the biosynthesis in Reader's medium. There was found higher yield on synthesis by cells harvested at the exponential phase than those at the stationary phase. The thiamine synthesized, was always found in cells, not in medium and about 1/4 of the thiamine was shown as its phosphorylated forms.

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (III) EFFECT OF THIAMINE ON BIOSYNTHESIS OF THIAMINE BY SACCHAROMYCES CEREVISIAE PREINCUBATED WITH THIAMINE

Article type: Article
1969 Volume 40 Issue 2 Pages 67-71
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_67

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (III) EFFECT OF THIAMINE ON BIOSYNTHESIS OF THIAMINE BY SACCHAROMYCES CEREVISIAE PREINCUBATED WITH THIAMINE

Article type: Article
1969 Volume 40 Issue 2 Pages 71-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_71_1

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (III) EFFECT OF THIAMINE ON BIOSYNTHESIS OF THIAMINE BY SACCHAROMYCES CEREVISIAE PREINCUBATED WITH THIAMINE

Article type: Article
1969 Volume 40 Issue 2 Pages 71-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_71_2

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ACCUMULATION AND BIOSYNTHESIS OF THIAMINE IN YEAST : (III) EFFECT OF THIAMINE ON BIOSYNTHESIS OF THIAMINE BY SACCHAROMYCES CEREVISIAE PREINCUBATED WITH THIAMINE

Chikataro KAWASAKI, Toshihiro IIO, Masaomi KONDO

Article type: Article
1969 Volume 40 Issue 2 Pages 71-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_71_3

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Biosynthesis of thiamine from its pyrimidine and thiazole moieties by Sacch. cerevisiae harvested in the medium containing thiamine or its pyrimidine and thiazole moieties, was shown to be more retarded than that by cells harvested in the medium without thiamine or with either pyrimidine or thiazole moiety. When the cells harvested in the medium without thiamine, had been incubated with thiamine for 1 or 3 hours before the reaction of biosynthesis, no retardation for biosynthesis was demonstrated. Biosynthesis of thiamine by crude enzyme extracted from cells harvested in the thiamine-containing medium was also proved to be more retarded and less phosphorylated than that from cells in thiamine-free medium. Under the retarded biosynthesis of thiamine by yeast cells, it was shown that uptake of the pyrimidine moiety by cells from the medium was also repressed.

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STUDIES ON FATTY ACID ESTERS OF FLAVINS : (XVIII) DECOLORATION OF RIBOFLAVIN TETRABUTYRATE UPON IRRADIATION IN OIL

Yoshiko YAMAMOTO, Kunio YAGI

Article type: Article
1969 Volume 40 Issue 2 Pages 72-75
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_72

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Riboflavin tetrabutyrate was dissolved in fresh soybean oil to 50 μg/ml, which was irradiated with Mazda daylight lamp. After irradiation for 4 hours, the absorbance at 445 mμ was decreased to 41% of the original absorbance, but the decrease was recovered up to 56% by mere storage in the dark for 48 hours. However, when the solution was irradiated for 10 hours, this recovery remained only 26%. These results suggest that riboflavin tetrabutyrate dissolved in the oil can be partly reduced by irradiation and reversed through slow oxidation. The irreversible reaction could be attributed to the decomposition of the isoalloxazine nucleus of riboflavin tetrabutyrate due probably to its reaction with lipoperoxide produced in the oil upon irradiation.

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FOLIC ACID DEFICIENCY DUE TO ANTICONVULSANT DRUGS

Hiroshi SANADA, Hirokuni TAGUCHI

Article type: Article
1969 Volume 40 Issue 2 Pages 83-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_83_4

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Twenty-five inpatients of a psychiatric clinic showed high incidence of the sign of folic acid deficiency. Low serum folate levels (L. casei assay) was revealed in 64% of the patients and macrocytosis (MCV over 95μ) in 64%. Hypersegmented polymorphs were increased in 24%. Mean diameter of the nucleus of the oral epithelial cells were a little greater than normal. On the other hand, serum iron levels, formiminoglutamic acid excretion after histidine loading and myelogram were normal. After the oral administration of 5mg of folic acid for 30 days, macrocytosis and hypersegmented polymorphs disappeared. The growth of L. casei or L. leichmannii was not suppressed in vitro by addition of various anticonvulsants in the medium.

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SEPARATION OF FAT-SOLUBLE VITAMINS BY GEL FILTRATION : (II) SEPARATION AND DETERMINATION OF VITAMIN A AND α-TOCOPHEROL

Fumio UEDA, Teruo MAKINO, Youko TOYOHIRA

Article type: Article
1969 Volume 40 Issue 2 Pages 84-90
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_84

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A method for the separation and determination of vitamin A and α-tocopherol in mixtures by gel filtration using the column of Sephadex LH-20 was examined. Hydrolysis of the samples was carried out in ethanolic potassium hydroxide by addition of antioxidants. The unsaponifiable fraction was extracted with ether in usual method. The extracted vitamin A and α-tocopherol were fractionated on Sephadex LH-20 column as the gel bed and chloroform as the eluting solvent. After treatment of gel filtration vitamin A and α-tocopherol were determined using the spectrophotometrric assay for vitamin A and Emmerie・Engel assay for α-tocopherol. Separation of vitamin A and α-tocopherol was achieved successfully by this method and recoveries were satisfactory.

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STUDIES ON THIAMINE AUXOTROPHS OF ESCHERICHIA COLI : OXIDATION OF 2-OXOGLUTARATE IN A MUTANT STRAIN WHICH REQUIRES EITHER THIAMINE OR SUCCINATE

Article type: Article
1969 Volume 40 Issue 2 Pages 96-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_96_1

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STUDIES ON THIAMINE AUXOTROPHS OF ESCHERICHIA COLI : OXIDATION OF 2-OXOGLUTARATE IN A MUTANT STRAIN WHICH REQUIRES EITHER THIAMINE OR SUCCINATE

Hideo NAKAYAMA, Yoko MATSUOKA

Article type: Article
1969 Volume 40 Issue 2 Pages 96-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_96_2

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Cell-free extracts of a mutant strain of Escherichia coli, W2-3,which required either thiamine or succinate for aerobic growth, and of its parent strain, W, were compared for their ability to catalyze overall oxidation of 2-oxoglutarate. Both extracts catalyzed the reduction of NAD in the presence of 2-oxoglutarate, CoA, and thiamine diphosphate, though the former was less active than the latter. Subsequently, both extracts catalyzed the anaerobic evolution of carbon dioxide from 2-oxoglutarate in the presence of ferricyanide as electron acceptor with variable amounts of thiamine diphosphate. The rate of carbon doixide evolution was always higher in the extracts prepared from the wild type strain than those prepared from the mutant strain at the same concentration of thiamine diphosphate. These results suggest that the biochemical lesion which was altered genetically in the mutant strain was at a stage in the decarboxylation of 2-oxoglutarate and that the stage was not blocked completely but the enzymic properties might be changed and became to require thiamine diphosphate in excess.

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CHANGE OF LIPID CONTENT IN RAT SERUM AFTER ADMINISTRATION OF RIBOFLAVIN TETRANICOTINATE

Toru ADACHI, Masato ASAHINA, Toru SHIINA

Article type: Article
1969 Volume 40 Issue 2 Pages 100-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_100_3

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After intraperitoneal injection of nicotinic acid, FMN and riboflavin tetranicotinate (B_2-Nic) into rats, the amounts of free fatty acid, triglyceride, phospholipid and cholesterol in the serum were studied on time schedule. Free fatty acid was decreased in a short time after the injection of nicotinic acid and B_2-Nic, though the decrease by B_2-Nic seemed to be somewhat delayed, but it again increased to the higher value than the normal, 8 hours after the injection. On the contrary, it was increased in a short time after the injection of FMN. Triglyceride was markedly decreased by nicotinic acid and B_2-Nic, and considerably by FMN. Cholesterol and phospholipid was also decreased by all these compounds to some extent.

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EFFECT OF AUTONOMIC BLOCKING AGENT ON THE RIBOFLAVIN METABOLISM IN THE SALIVARY GLAND

Kijuro OBARA, Shigeru ONO, Yasubumi TAKAHASHI, Yoshio KUSAKA

Article type: Article
1969 Volume 40 Issue 2 Pages 103-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_103

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The change in riboflavin metabolism of dog's salivary glands was investigated when autonomic blocking agents were administered subcutaneously. The contents of total riboflavin in the three salivary glands were found to increase at 30 minutes and to decrease than normal levels at 60 minutes after an administration of atropine. Total riboflavin content decreased in the stage of hypersecretion in parotid and submandibular gland by administration of pilocarpine, whereas it increased in the sublingual gland. Decrease of FAD and increases of FMN and free riboflavin recognized in the three salivary glands by administration of atropine. FAD fraction in the parotid increased characteristically at 30 minutes after administration of pilocarpine. From these results, the authors suggest that there is a close correlation between salivary secretion and riboflavin metabolism, furthermore, that the parotid and the submandibular glands are similar organs functionally, because of similar response was found in these organs for the administration of autonomic blocking agents.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 104-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_104_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1969 Volume 40 Issue 2 Pages 104-105
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_104_2

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 105-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_105_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 106-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_106_3

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 107-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_107_1

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Article type: Article
1969 Volume 40 Issue 2 Pages 107-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_107_2

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[title in Japanese]

[in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 107-108
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_107_3

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[title in Japanese]

[in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 108-109
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_108

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 109-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_109_1

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 109-110
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_109_2

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Article type: Article
1969 Volume 40 Issue 2 Pages 110-111
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_110

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 111-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_111

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Article type: Article
1969 Volume 40 Issue 2 Pages 112-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_112_1

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 112-113
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_112_2

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 113-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_113_1

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 113-114
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_113_2

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Article type: Article
1969 Volume 40 Issue 2 Pages 114-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_114_1

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Article type: Article
1969 Volume 40 Issue 2 Pages 114-115
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_114_2

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 116-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_116_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 116-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_116_2

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[title in Japanese]

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Article type: Article
1969 Volume 40 Issue 2 Pages 116-117
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_116_3

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[title in Japanese]

[in Japanese]

Article type: Article
1969 Volume 40 Issue 2 Pages 117-
Published: August 25, 1969
Released on J-STAGE: February 22, 2018

DOIhttps://doi.org/10.20632/vso.40.2_117_2

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