VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
DETERMINATION OF VITAMIN D BY THIN-LAYER CHROMATOGRAPHY : (I) THIN-LAYER CHROMATOGRAPHY OF VITAMIN D AND RELATFD COMPONDS
Akiji FUJITASachiko TOKUHISAKatsuko MICHINAKA
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1969 Volume 40 Issue 3 Pages 121-128

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Abstract
(1) Quantitative determination of vitamin D by alumina or silicagel thin-layer chromatography (TLC) using benzene・acetone・chloroform (10 : 1 : 1) as a developing solvent system is possible in the range of 5 to 50μg and the necessary conditions were described. (2) Absorption spectra in ultraviolet region of vitamin D and related compounds and those of the colored solutions by Nield reagent were determined. No correlations were found between the two. (3) Those showing the absorption spectra of the Nield reaction similar to that of vitamin D were isoergocalciferol, isotachysterol_2,tachysterol_2 and 5,6-trans ergocalciferol. (4) In the chromatogram of TLC those having the Rf values near that of vitamin D were tachysterol_2,isotachysterol_2,5,6-trans ergocalciferol, isoergocalciferol, retinol, ergosterol, 7-dehydrocholesterol, cholesterol, and lumisterol. Of these compounds, cholesterol, ergosterol and 7-dehydrocholesterol were completely removed by digittonin treatment. (5) Of these compounds, the one having the most effect on the determination of vitamin D is tachysterol. It seems to be somewhat decomposed by digitonin treatment and TLC but it will be difficult to be fractionated from vitamin D in the presence of much tachysterol.
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© 1969 THE VITAMIN SOCIETY OF JAPAN

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https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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