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Volume 40 (1969)
- Issue 6 Pages 397-
- Issue 5 Pages 295-
- Issue 4 Pages 219-
- Issue 3 Pages 121-
- Issue 2 Pages 59-
- Issue 1 Pages 1-
Volume 40, Issue 3
Displaying 1-18 of 18 articles from this issue
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Akiji FUJITA, Sachiko TOKUHISA, Katsuko MICHINAKAArticle type: Article
1969 Volume 40 Issue 3 Pages 121-128
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESS(1) Quantitative determination of vitamin D by alumina or silicagel thin-layer chromatography (TLC) using benzene・acetone・chloroform (10 : 1 : 1) as a developing solvent system is possible in the range of 5 to 50μg and the necessary conditions were described. (2) Absorption spectra in ultraviolet region of vitamin D and related compounds and those of the colored solutions by Nield reagent were determined. No correlations were found between the two. (3) Those showing the absorption spectra of the Nield reaction similar to that of vitamin D were isoergocalciferol, isotachysterol_2,tachysterol_2 and 5,6-trans ergocalciferol. (4) In the chromatogram of TLC those having the Rf values near that of vitamin D were tachysterol_2,isotachysterol_2,5,6-trans ergocalciferol, isoergocalciferol, retinol, ergosterol, 7-dehydrocholesterol, cholesterol, and lumisterol. Of these compounds, cholesterol, ergosterol and 7-dehydrocholesterol were completely removed by digittonin treatment. (5) Of these compounds, the one having the most effect on the determination of vitamin D is tachysterol. It seems to be somewhat decomposed by digitonin treatment and TLC but it will be difficult to be fractionated from vitamin D in the presence of much tachysterol.View full abstractDownload PDF (780K) -
Akiji FUJITA, Sachiko TOKUHISA, Katsuko MICHINAKA, Tadayoshi ONO, Wata ...Article type: Article
1969 Volume 40 Issue 3 Pages 129-135
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESS(I) The various conditions for detemining vitamin D by thin-layer chromatography in Lentinus ededes were studied and the appropriate conditions were described. (2) The vitamin D contents of commerical dried fungus preparations were found to be in the range of 19 to 100 I.U./g of vitamin D. The contents less than 40 I.U./g could hardly be determined owing to the contaminations of much noncalciferol compounds as compared with those of vitamin D. The commercial fresh fungus contained the vitamin in the order of 20 I.U. per g dry weight. (3) After irradiation of the slices of the fungus with the "Erythemal Fluorescent Lamp", the content of viamin D increased with the time of irradiation. The content of the vitamin rose from 100 I.U./g before irradiation to 3000 I.U./g after irradiation for 120 minutes. (4) After irradiation of the under surface of the fungus with "Hitachi Mercury Lamp" at a distance of 10 cm, the vitamin rose from 10 to 50μg/g in 60 minutes and those of pre-D from 10 to 30 μg/g, whereby the ratio of pre-D to the sum of vitamin D and pre-D rose to 37 per cent in 60 minutes.View full abstractDownload PDF (692K) -
Yasuhiro YAMANE, Motoichi MIYAZAKI, Yohko YAMADA, Masakatsu OHTAWAArticle type: Article
1969 Volume 40 Issue 3 Pages 136-141
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe previous paper had shown that the red fluorescent product was formed by reaction of thiolized-thiamine with cuprous ion, and it could be applied to the analysis of thiamine in spot test and extraction method. In this paper, a new method for the fluorometric determination of thiamine with copper was established. The cuprous ion reagent was prepared by reduction of cupric sulfate with hydroxylamine sulfate, and adjusting pH to 7.2-7.8. The sample solution of thiamine was thiolized with sodium hydroxide solution at pH 11.0-12.0,and then heated for 30 seconds at 70℃. The reaction product of cuprous-thiolized thiamine system was extracted with isoamyl alcohol containing 30 μg/ml isoamyl gallate as a stabilizer, and then intensity of red fluorescence of isoamyl alcohol layer at 640mμ excited by 320mμ rays was then measured. The working curve of thiamine in this method was almost straight for 2-30 μg/ml. This determination was interfered with Hg^<2+>, Na_2S, oxine, sulfathiazole and riboflavin.View full abstractDownload PDF (444K) -
Hirobumi OHAMA, Kunio YAGIArticle type: Article
1969 Volume 40 Issue 3 Pages 142-148
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSUnder the condition of light irradiation the reaction of riboflavin-2', 3', 4', 5'-tetrabutyrate with linoleic acid (or methyl linoleate) was investigated. The results revealed that the formation of linoleic acid peroxide was markedly inhibited in the presence of riboflavin tetrabutyrate. Absorbance at 447 mμ (E_<447>) of B_2-butyrate dissolved in linoleic acid was decreased by light irradiation, but this decrease was partially recovered by standing the solution in the dark. Since this recovery of E_<447> was partial and not complete, it was considered that at least two processes, viz. reversible and irreversible processes, were included. The reversible decrease of E_<447> of B_2-butyrate is probably due to the photoreduction of B_2-butyrate upon irradiation. The irreversible decrease of E_<447> of B_2-butyrate may be attributed to the decomposition of the isoalloxazine nucleus of B_2-butyrate caused by its reaction with linoleic acid in the process of the peroxide formation.View full abstractDownload PDF (663K) -
Yoshio KATO, Akira HATANOArticle type: Article
1969 Volume 40 Issue 3 Pages 149-151
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSStability of ascorbic acid monostearate was investigated. Samples were prepared to have pH of 2.0-9.0,with Britton・Robinson buffer solution and were irradiated and the rates of decomposition were determined by polarography. Ascorbic acid monostearate is relatively more stable than free ascorbic acid in alkaline state.View full abstractDownload PDF (303K) -
Chikataro KAWASAKI, Toshihiro IIO, Masaomi KONDOArticle type: Article
1969 Volume 40 Issue 3 Pages 152-156
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe inhibitory effect of thiamine analogues and pyrimidine compounds, shown in Fig. 1,on the intracellular accumulation of thiamine was investigated by using Sacchromyces cerevisiae. Phenylthiazinothiamine (PTT) was proved to be most effective to inhibit the accumulation of thiamine, although PTT was not an antagonist of thiamine. PTT was shown to have no inhibitition on both growth of the yeast and ethanol production during the growth at the corresponding concentration (see Fig. 4,5). From the above results, it will be concluded that PTT has a very high potency to block of the accumulation of thiamine on yeast cells without any other metabolic inhibition to cells.View full abstractDownload PDF (516K) -
Chikataro KAWASAKI, Toshihiro IIO, Masaomi KONDOArticle type: Article
1969 Volume 40 Issue 3 Pages 157-160
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSPhenylthiazinothiamine (PTT) was shown to have no inhibition of thiamine biosynthesis from its pyrimidine and thiazole moieties : 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP) and 4-methyl-5-(β-hydroxy) ethylthiazole (MHT), by Saccharomyces cerevisiae. Biosynthesis of thiamine by yeast cells with PTT even in the broth containing 50μg of thiamine was proved to be completed without absorption of the thiamine from the broth. The standard assay method suggested by the authours is as follows. Cells of Sacch. cerevisiae cultured in Atkin's medium (16hrs. at 30℃) were harvested and suspended in 15 ml of Reader's medium (O.D. 1.0). After addition of MHT (1μmole), PTT (1 μmole) and OMP (0.01〜0.1 μmole) or sample to the suspension, it was incubated for 3 hrs. at 30℃ and the thiamine content in the centrifuged yeast cells was determined by thiochrome method.View full abstractDownload PDF (425K) -
Yoshitomo KOCHI, Keizo NAKAMURA, Sumiko UEOKAArticle type: Article
1969 Volume 40 Issue 3 Pages 161-165
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSFor isolating the reaction products in this coloration, an attempt was made to use 6-O-benzoylascorbic acid instead of ascorbic acid and a stable blue dye soluble in organic solvent was obtained. To elucidate the structure, this dye was examined by elemental analysis, molecular weight determination, infrared spectroscopy and fluorescent reaction with o-phenylenediamine. From the findings of these examinations, it was presumed that the dye had the chemical etructure of indophenine type in which 2 moles of pyrrole combined at α-position with carbonyl group at 3-position of 2 moles of 6-O-benzoyldehydroascorbic acid.View full abstractDownload PDF (447K) -
Yoshitomo KOCHI, Keizo NAKAMURA, Sumiko UEOKAArticle type: Article
1969 Volume 40 Issue 3 Pages 166-169
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe coloration of ascorbic acid derivatives with pyrrole was studied, and was found that 5,6-diacetylascorbic acid oxidized with iodine produced a blue color with pyrrole, while acetylated dehydroascorbic acid, derived from acetylation of dehydroascorbic acid, unexpectedly did not. On the supposition that this peculiar phenomenon was due to the specific structure of acetylated dehydroascorbic acid, various investigations were performed to elucidate that above acetylated dehydroascorbic acid was a dimer identical with tetraacetyl-bis-dehydroascorbic acid obtained by H. Albers, et al. by acetylating bis-dehydroascorbic acid. Further studies with mass-, infrared- and NMR-spectra did not reveal any fact that deny the proposed structure by H. Albers. In the above dimer, 2 moles of dehydroascorbic acid combined at 2,3'-positions and 2,3'-positions respectively, forming a dioxane ring. These facts would further support the presumed mechanism that the coloration with pyrrole was due to the participation of carbonyl group at 3 position of dehydroascorbic acid, as reported in a preceding paper.View full abstractDownload PDF (463K) -
Masahiro KAWASAKI, Kazuaki FUJIMOTO, Tomiyoshi ITO, Yoshiaki YAMASHITAArticle type: Article
1969 Volume 40 Issue 3 Pages 170-174
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAs previously reported, no free inosine can be detected in serum of normal rabbit, but the compound appears when the animal was invaded by antigen. Content of inosine in blood increased gradually after the first sensitization of typhoid-paratyphoid vaccine, an antigen, especially after the second sensitization, and reached to maximum value (34.0±4.5μg/ml) at 2 hours later. Injection of a large quantity of biotin (5 mg/kg) prior to the second sensitization, however, resulted that the free inosine was hardly detected in blood. Normal liver homogenate was able to deaminate adenosine to inosine. The deamination, however, was disturbed by adding of biotin. Successively, time course of biotin content in blood was estimated. Normal rabbit serum contained biotin 18.8±3.8mμg/ml. When biotin was injected to both normal rabbits and those preinjected with typhoid-paratyphoid vaccine, biotin in blood decreased after high value in normal ones within 6 hours, in preinjected ones, however, resulted durable high content of biotin.View full abstractDownload PDF (484K) -
Masahiro KAWASAKI, Kazuaki FUJIMOTO, Tomiyoshi ITO, Yoshiaki YAMASHITAArticle type: Article
1969 Volume 40 Issue 3 Pages 175-178
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSFree biotin in blood was microbiologically assayed according to Skeggs・Wright. Total content of biotin was estimated after hydrolysis with 3 N H_2SO_4 for 30 minutes at 120℃. Biotin content in normal rabbit serum was 19.5 mμg/ml as total, and about 98.4% of the total was free biotin. After second sensitization with typhoid-paratyphoid vaccine, biotin content in serum was decreased remarkably and reached about 77% of normal value at two hours later, since inosine content was increased at the time as previously reported. When a large quantity of inosine (5 mg/kg) was preinjected between first and second sensitization with typhoid-paratyphoid vaccine, biotin content in serum did not vary with the second sensitization. These results suggested that the two compounds, biotin and inosine, were corelated in metabolic control system of rabbit liver.View full abstractDownload PDF (407K) -
Taro NAKATAArticle type: Article
1969 Volume 40 Issue 3 Pages 179-184
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSUnidentified six biologically active lipoic acid-like substances showing varing Rf values on paper chromatogram with a solvent of n-butanol saturated with 0.5N ammonium hydroxide beside α- and β-lipoic acids were detected in human urine. Main activities on the bioautogram were observed on the spots corresponded to Rf 0.02 and 0.12 which responded to Corynebacterium bovis, but not Streptococcus faecalis and were tentatively named as S_1-and S_2-compounds, respectively. These substances were partly soluble in organic solvents such as ethylacetate and butanol, but hardly soluble in chloroform and benzene at acidic pH. They were easily convertable to α-lipoic acid responsible for Streptococcus faecalis by acid hydrolysis. The uriary excretion of ^<35>S-metabolic compounds by rats was investigated following intraperitoneal injection of 20 mg/kg of DL-lipoic acid-^<35>S. About 80% of radioactivity was recovered in 24hr urine. The radioactivities on radioautogram were observed on the spots corresponding to the microbiological growth, but the amounts of radioactivity appearing on radioautogram were considerably larger than those of the microbiologically active substances on bioautogram. This suggested that ^<35>S-metabolic compounds excreted in rat urine contained the biologically inactive metabolite of lipoic acid-^<35>S.View full abstractDownload PDF (629K) -
Taro NAKATAArticle type: Article
1969 Volume 40 Issue 3 Pages 185-192
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSMain biologically active lipoic acid-like substances, which are tentatively named as S_1- and S_2-compounds, were isolated from the human urine following the administration of lipoamide. Both compounds were separated from lipoic acid and other lipoic acid-like substances in the urine by extraction with organic solvents, treatment with ion exchange resins and silica gel column chromatography. It was confirmed that the S_1- and S_2-compounds are derived from ^<35>S-lipoic acid administered to rats. Further, S_2-compound which was larger than S_1,was separated from the mixture by paper chromatography. It responded to Corynebacterium bovis but not Streptococcus faecalis and easily converted to lipoic acid by acid hydrolysis. At the same time it released four ninhydrin-positive compounds on paper chromatogram. By paper electrophoresis the S_2-compound migrated to the anode easier than lipoic acid at a range of pH 3-4. It was also found not to be a simple lipoamide derivative because of stability to lipoamidase which was partially purified from Corynebacterium bovis. The chemical structure of S_2-compound was discussed.View full abstractDownload PDF (821K) -
Taro NAKATAArticle type: Article
1969 Volume 40 Issue 3 Pages 193-198
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe daily urinary excretion of lipoic acid and urinary excretion after administration of lipoamide were compared, determining by microbiolgical assay between normal adults and hospitalized patients of liver diseases. The total lipoic acid contents in the urine were determined after hydrolysis by autoclaving for 1 hour with 6N HCl under nitrogen and in the presence of bovine serum albumin. The following results were obtained. No significant differences were found in the daily excretion of total lipoic acid between the normal and the patients, however the excretion of total lipoic acid in 24 hr urine after oral administration of lipoamide (30 mg) was larger on average in the normal adults (980 μg) than in the patients (698 μg). This difference was caused by the decrease (about 50% of the normal controls) of total lipoic acid in the 3 hr urine of the patients after administration.View full abstractDownload PDF (625K) -
Kijuro OBARA, Shigeru ONO, Yoshio KUSAKA, Shinta FUKUIArticle type: Article
1969 Volume 40 Issue 3 Pages 199-201
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSEffect of autonomic blocking agent on the thiamine distribution in the salivary gland of dog was investigated by means of thiochrome method. Total and free thiamines in the parotid and submandibular gland were found to be decreased at 30 minutes after atropine sulfate injection subcutaneously and then they were restored to normal value gradually, whereas it was found to increase remarkably at 30 minutes after pilocarpin hydrochloride injection. From these results the authors suggest that there are close correlations between salivary secretion function and thiamine metabolism and that thiamine metabolism is promoted remarkably when salivary gland was hyperfunctional state, on the contrary, decrease of salivary secretion was induced the inhibition of thiamine turnover in the gland.View full abstractDownload PDF (364K) -
Ichiro KUROKAWA, Toshiyuki KIMURA, Yoshiko UENO, Keiko NAGAHAMA, Iho S ...Article type: Article
1969 Volume 40 Issue 3 Pages 202-205
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe influence of vitamin E on the osmotic fragility of erythrocyte and leucocyte counts was investigated. The blood samples were obtained from normal persons in citrate・phosphate・dextrose solution or in EDTA-K_3. α-Tocopherylglycolate was added with the concentration of 20 or 50 mg%. The osmotic fragility was measured by Dacie's method and leucocyte count by micro cell counter, once or twice a week for 3 to 4 weeks. The osmotic fragility was evidently increased and leucocyted and leucocytes showed decrease in control and tocopherol groups, but it was less marked in the latter. The protective potency of the increase of osmotic fragility was more marked in 50 mg % than 20 mg % of α-tocopherylglycolate, and its potency was almost equal comparing with that of α-tocopherylacetate.View full abstractDownload PDF (356K) -
Ichiro KUROKAWA, Toshiyuki KIMURA, Yoshiko UENO, Keiko NAGAHAMA, Iho S ...Article type: Article
1969 Volume 40 Issue 3 Pages 206-209
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe influence of vitamin E on the morphological preservation of erythrocytes was investigated. Normal human blood in citrate・phosphate・dextrose was diluted with its own plasma and classified microscopically into 4 types (I : biconcave disc form, II : slight crenated form, III : evident crenated form, IV : swollen, oval form). α-Tocopherylacetate was added with the concentration of 10 or 20 mg%, and in the latter case, more erythrocytes remained normal (type I) than in 10 mg% or control cases at the 7th day after sampling. No remarkable difference was existed between 10 mg% and control cases with solvent, in the respect of residual rate of normal erythrocytes. In both cases, to which α-tocopherylglycolate were added with the concentration of 20 or 50 mg% respectively, more normal erythrocytes were observed comparing with control cases with or without solvents.View full abstractDownload PDF (889K) -
Yasuho NISHII, Nobuhiko KATUNUMA, Ikuko TOMINOArticle type: Article
1969 Volume 40 Issue 3 Pages 216-
Published: September 25, 1969
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSEffects of pyrithioxine, a pyridoxine derivative, on the GOTM shuttle and on the activities of enzymes concerned with the metabolism of γ-aminobutyrate, glutamate, glutamine and cystathionine were investigated. The interconversions of glutamate into aspartate and of aspartate into glutamate with rat brain mitochondria were not affected by the presence of 10^<-4>M of pyrithioxine. Pyrithioxine in 10^<-4>M had no effects on the activities of rat brain glutamic decarboxylase, pig brain γ-aminobutyrate transaminase, rat brain glutaminase and glutamine synthetase. With partially purified preparation from rat brain, it was succeeded to detect the activity of cystathionase. The activity of the enzyme was not effected by pyrithioxine in various range of homoserine concentrations. Pyrithioxine did not effect in any concentration to the activities of pyridoxal phosphate as coenzyme for apoglutamic decarboxylase of rat brain and apocystathionase of rat liver.View full abstractDownload PDF (148K)
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