Abstract
The inhibitory effect of thiamine analogues and pyrimidine compounds, shown in Fig. 1,on the intracellular accumulation of thiamine was investigated by using Sacchromyces cerevisiae. Phenylthiazinothiamine (PTT) was proved to be most effective to inhibit the accumulation of thiamine, although PTT was not an antagonist of thiamine. PTT was shown to have no inhibitition on both growth of the yeast and ethanol production during the growth at the corresponding concentration (see Fig. 4,5). From the above results, it will be concluded that PTT has a very high potency to block of the accumulation of thiamine on yeast cells without any other metabolic inhibition to cells.
