Abstract
As previously reported, no free inosine can be detected in serum of normal rabbit, but the compound appears when the animal was invaded by antigen. Content of inosine in blood increased gradually after the first sensitization of typhoid-paratyphoid vaccine, an antigen, especially after the second sensitization, and reached to maximum value (34.0±4.5μg/ml) at 2 hours later. Injection of a large quantity of biotin (5 mg/kg) prior to the second sensitization, however, resulted that the free inosine was hardly detected in blood. Normal liver homogenate was able to deaminate adenosine to inosine. The deamination, however, was disturbed by adding of biotin. Successively, time course of biotin content in blood was estimated. Normal rabbit serum contained biotin 18.8±3.8mμg/ml. When biotin was injected to both normal rabbits and those preinjected with typhoid-paratyphoid vaccine, biotin in blood decreased after high value in normal ones within 6 hours, in preinjected ones, however, resulted durable high content of biotin.
