Abstract
Reaction of riboflavin-5'-phosphate with butyric anhydride in pyridine affored 2', 3'-di-O-butyrylriboflavin-4', 5'-cyclic phosphate (Ib) in a good yield. Acid hydrolysis of Ib gave a mixture of 2', 3'-di-O-butyrylriboflavin-4'-phosphate (IIb) and -5'-phosphate (IIIb). By similar methods, dibenzoyl and dinicotinoyl derivatives of riboflavin-4'(5')-phosphate are also prepared. These diacylriboflavin-4'(5')-phosphates are readily hydrolyzed by acid- and alkaline phosphatases, whereas hardly hydrolyzed by hog pancreatic lipase.
