VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
Search
Advanced search
OR
Browse
Search
Volume 40, Issue 6
Displaying 1-25 of 25 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • METABOLIC ERROR OF FOLIC ACID
    Tsuneo ARAKAWA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 397-401
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_397
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    As inborn errors of folic acid metabolism, formiminotransferase deficiency and N^5-methyltetrahydrofolate transferase deficiency were reported. Clinical features common to both syndromes were mental retardation, abnormalities in EEG and PEG which were considered to be resulted from an impaired purine biosynthesis of the growing brain due to metabolic error of folic acid. That folic acid deficiency in early infancy caused a delay in maturation patterns of the EEG basic waves was demonstrated by experimental and clinical studies.
    View full abstract
    Download PDF (445K)
  • STUDIES ON THE PANTOTHENIC ACID CONTENT IN SERUM AMONG VILLAGERS OF IWATE PREFECTURE
    Ken SAITO, Shinichi HAREYAMA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 402-404
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_402
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The difference of the pantothenic acid content in serum and of urinary sodium excretion between persons of hypertensive and of normal blood pressure was investigated. The pantothenic acid content (μg/dl) in serum of people in normal and high blood pressure was 11.3〜14.8 and 8.8〜12.6,respectively. Several factors have been discussed on the cause of hyperpiesia and atherosclerosis, but our investigation showed that there was a pantothenic acid deficiency in serum of the hypertensive and that the pantothenic acid deficiency induced the decrease of urinary sodium excretion.
    View full abstract
    Download PDF (352K)
  • FUNDAMENTAL STUDIES OF MICROBIOLOGICAL ASSAY FOR VITAMIN B-COMPLEX BY CUP-PLATE TECHNIQUE : (XV) A MICROBIOLOGICAL ASSAY METHOD FOR PANTOTHENIC ACID BY CUP-PLATE TECHNIQUE USING LACTOBACILLUS ARABINOSUS
    Masako YAMADA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 405-411
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_405
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The cup-plate assay method for pantothenic acid with Lactobacillus arabinosus as test organism, in the case of a pure solution of the vitamin was satisfactory enough in the specificity, precision and response range, though it was relatively insensitive. However, when the assay solutions for total pantothenic acid from several animal and vegetable samples obtained both by an enzymatic treatment and by a boiling water extraction were used, it was almost unable to determine the net content of the vitamin, because of a presence in these samples of certain factors affecting the growth zone responses of the test organism. Thus, so far as the present preparations of pantothenic acid from natural products are employed, the valid and reliable assay of the vitamin by the plate technique cannot be expected.
    View full abstract
    Download PDF (1158K)
  • STUDIES ON THE DISTRIBUTION OF PANTOTHENIC ACID, COENZYME A AND THEIR INTERMEDIATES IN RAT LIVER : (III) THE ISOLATION OF PANTOTHENIC ACID. 4'-PHOSPHOPANTETHEINE AND COENZYME A BY USING COLUMN CHROMATOGRAPHY
    Tsuneo NAKAMURA, Tomoichi KUSUNOKI, Kokichi SOYAMA, Masaomi KUWAGATA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 415-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_415
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Pantothenic acid, CoA and their intermediates in rat liver were isolated by using DEAE-cellulose column chromatography and identified by means of paper chromatography. In rat liver CoA exsisted in the largest amounts and 4'-phosphopantetheine in second order. Pantothenic acid was recognized in very small amounts from this experiment. By the addition of CoA to rat liver sample, it was confirmed that very small amounts of 4'-phosphopantetheine was released by decomposition of CoA during the preparation of rat liver sample, but all 4'-phosphopantetheine present in preparation did not mean to derive from decomposition of CoA alone. In our future study the relation between 4'-phosphopantetheine and acyl carrier protein would be investigated.
    View full abstract
    Download PDF (166K)
  • STUDIES ON THE ASCORBIC ACID AND ITS COMPLEXES : (V) BEHAVIOR OF COPPER ION TO ASCORBIC ACID
    Akira HATANO, Yoshio KATO
    Article type: Article
    1969 Volume 40 Issue 6 Pages 419-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_419_2
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    When copper chloride was added to an aqueous ascorbic acid solution and the behavior of copper inons was polarographed, it was found that the half-wave potential of the copper ions was -0.14 V vs. SCE. It is thougt that the Cu^<2+> is first reduced to Cu^+ and then chelate comounds are formed. The UV spectrum of the sample showed absorption banbs at λ^<H_2O>_<max> 720 mμ and 798 mμ, and in contrast to the UV spectrum of ascorbic acid (λ^<H_2O>_<mex> 245 mμ) it had shifted toward much longer wave lengths. A study of the electron transition mechanism was made when a copper chelate compund of ascorbic acid is formed and this shows that activity coefficients (kf_0) will be 3.02×10^<-3> for pH 6,3.27×10^<-3> for pH 8,and 2.83×10^<-3> for pH 9. No significant changes can be observed in the activation energy and activation entropy obtained from these activity coefficients for the samples at an electrolyses temperature of 25℃. This implies that during activation in the electric field of the electrode the contributing effect of induced dipole efficiency is greater than that of permanent dipole efficiency of the chelate itself. Because ascorbic acid is oxidized by the copper ions and dehydroascorbic acid forms copper chelate compounds, a phenomenon will be observed in which the characteristic absorption band on the UV spectrum shifts toward the visible region and the half-wave potential of Cu^+ becomes positive simultaneously.
    View full abstract
    Download PDF (152K)
  • STUDIES ON SORBIC ACID : (VIII) ON THE REMARKABLE INHIBITION OF BAKER'S YEAST RESPIRATION WITH CO-EXISTENCE OF SORBIC ACID AND THIAMINE
    Ryoichi HIGUCHI, Kiyoshi HARADA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 420-423
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_420
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    In the previous report, it was confirmed that the respiration of baker's yeast was stimulated by the addition of a large amount of thiamine and was inhibited with sorbate, however, the co-existence of thiamine and sorbate had a significant inhibitory effect on the respiration. In the present report, the mechanism of the remarkable inhibition is studied. The addition of a large amount of thiamine in the growth medium resulted the decrease of CoA level in the cells and the CoA synthesizing ability of the enzymes, extracted from the cells, showed significant decrease compared with the control cells. At in vitro CoA synthesizing enzyme reaction, the addition of a large amount of thiamine also caused the decrease of the CoA formation and major part of the inhibition would be attributed to that of pantothenate kinase. From above results the possible mechanism of the remarkable inhibition is considered that the shortage of CoA on account of the formation of sorbyl-CoA and the decrease of the supplement of CoA with thiamine result the inhibition of the respiration.
    View full abstract
    Download PDF (432K)
  • STUDIES ON RIBOFRAVIN DIESTER : (III) METABOLIC ACTIVITIES OF RIBOFRAVIN-DINICOTINATE AND 3-N-BENZOYLRIBOFLAVIN IN RATS
    Shoichi MASUSHIGE, Saburo TAKANO, Takao SUZUKI, Yoshikazu SAHASHI
    Article type: Article
    1969 Volume 40 Issue 6 Pages 428-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_428_2
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    In the previous paper, it was reported that the effect of B_2-But_2^<*2)> had been investigated on rats. Afterwards, the present authors have studied the biological effects of synthetic riboflavin-dinicotinates on rats. Riboflavin-2', 3'-dinicotinate, riboflavin-4', 5'-dinicotinate and 3-N-benzoylriboflavin, that were newly synthesized by the Toa-Eiyo Chemical Industries Co. were used. Using riboflavin-deficient male rats of Wistar strain as test animals weighing 50- 60g, the prophylactic and curative experiments were carried out by oral administration with 30μg of each test compound as FR^<*6> / day / rat. The growth curves were recorded throughout the experimen's, and at the end of the experiments, animals were killed by decaptation and the total riboflavin contents of various tissues were estimated by the Yagi's fluorometric method, and the total nicotinic acid contents in the liver of the B_2-Nic^<*9> given animals were messured by the ordinary microbiological assay with L. ar abinosus. The results are briefly summarized as follows. The growth rates showed rather lower than that of the FR group, and the total nicotinic acid contents in the liver of B_2-Nic given animals was found to be remarkably increased.
    View full abstract
    Download PDF (149K)
  • STUDIES ON RIBOFLAVIN DIESTERS : (IV) PREPARATION AND ENZYMIC HYDROLYSIS OF DIACYLRIBOFLAVIN PHOSPHATES
    Teruo TANAKA, Hideo TANAKA, Toshitaka TSUBAKI, Mutsuo OSAWA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 433-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_433_2
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Reaction of riboflavin-5'-phosphate with butyric anhydride in pyridine affored 2', 3'-di-O-butyrylriboflavin-4', 5'-cyclic phosphate (Ib) in a good yield. Acid hydrolysis of Ib gave a mixture of 2', 3'-di-O-butyrylriboflavin-4'-phosphate (IIb) and -5'-phosphate (IIIb). By similar methods, dibenzoyl and dinicotinoyl derivatives of riboflavin-4'(5')-phosphate are also prepared. These diacylriboflavin-4'(5')-phosphates are readily hydrolyzed by acid- and alkaline phosphatases, whereas hardly hydrolyzed by hog pancreatic lipase.
    View full abstract
    Download PDF (154K)
  • FORMATION OF RIBOFLAVIN-α-GLUCOSIDE BY BLACK ASPERGILLUS
    Yukio SUZUKI, Kei UCHIDA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 434-438
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_434
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Experiments on the formation of riboflavin-α-glucoside from maltose and riboflavin were carried out with various strains of Aspergillus, Mucor, Penicillium and Rhizopus. It was observed that each bran culture extract of the molds Aspergillus awamori, A. kawachii, A. niger, A. saitoi, A. usamii and Mucor javanicus had a high ability to synthesize riboflavin-α-glucoside. Moreover, it was pointed out that a partially purified enzyme preparation of maltose α-1,4→1,6 transglucosidase prepared from shaking culture filtrate of A. awamori by the method of Okazaki had both riboflavin-α-glucoside-forming and -decomposing activities.
    View full abstract
    Download PDF (823K)
  • OCCURRENCE OF TWO RIBOFLAVINYLGLUCOSIDE FORMING ENZYMES FROM RIBOFLAVIN AND MALTOSE IN MUCOR JAVANICUS
    Sei TACHIBANA, Hideo IMATA
    Article type: Article
    1969 Volume 40 Issue 6 Pages 441-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_441
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The cell-free extract of Mucor javanicus was capable of riboflavinylglucoside formation from riboflavin and maltose. Two peaks of the riboflavinylglucoside producing activity were observed in 0.6 and 0.9 ammonium sulfate saturation fractions of the cell-free extract. The optimum pH of the former fraction was 4.5,and of the latter was 4.0. The substrate specificities of these two fractions were not coincident.
    View full abstract
    Download PDF (170K)
  • EFFECTS OF SOME SUBSTANCES ON THE COENZYME ACTIVITY OF PYRIDOXAL PHOSPHATE AND PYRIDOXAMINE PHOSPHATE TO APO-ENZYME OF CYTOPLASMIC ASPARTATE AMINOTRANSFERASE
    Yasuho NISHII, Toshiko SHIMIZU, Sohachi ANDO
    Article type: Article
    1969 Volume 40 Issue 6 Pages 448-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_448_2
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Asparate aminotransferase was isolated from the supernatant fraction of pig heart muscle according to the method of Morino, Itoh and Wada. Holoenzyme was resolved to apoenzyme and coenzyme by incubation with aspartate and phosphate, followed by chromatography with Sephadex column. Km values for α-ketoglutarate of holo- and apo-aspartate aminotransferases were 4×10^<-3>M and 2.5×10^<-4> M, respectively. With apo-aspartate aminotransferase, Lineweaver・Burk plots of the coenzyme concentration curves gave Km value of 1.33mμM for pyridoxal phosphate and 10mμM for pyridoxamine phosphate. The aspartate aminotransferase reaction was markedly inhibited by the preincubation of apoenzyme with pyridoxal phosphate or pyridoxamine phosphate in the presence of low concentration of α-ketoglutarate, but was not inhibited in the presence of other substrates, such as L-aspartate, L-glutamate or oxalacetate. This inhibition was typically competitive and Ki values for α-ketoglutarate were 2.6×10^<-6>M against pyridoxal phosphate and 6.28×10^<-5>M against pyridoxamine phosphate. Of these organic acids tested, such as pyruvate, citrate, malate, succinate, and lactate, only citrate inhibited competitively and its Ki values were calculated as 5.6×10^<-7>M against pyridoxal phosphate and 8.0×10^<-7>M against pyridoxamine phosphate from Lineweaver・Burk plots. Phosphate ion and some nucleotides also inhibited competitively, but pyridoxal and pyridoxamine did not inhibit. Ki values for phosphate were calculated as 2.15×10^<-7>M against pyridoxal phosphate and 3.9×10^<-7>M against pyridoxamine phosphate.
    View full abstract
    Download PDF (162K)
  • STUDIES ON THE ANTIVITAMIN B_6 ACTION ON LACTIC ACID BACTERIA AND YEAST : (V) INFLUENCE OF INOCULATING CONDITIONS ON THE GROWTHINHIBITION OF SACCHAROMYCES CARLSBERGENSIS 4228 BY THIAMINE
    Yasuo KAKIUCHI
    Article type: Article
    1969 Volume 40 Issue 6 Pages 449-453
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_449
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Influence of inoculating conditions on growth of Saccharomyces carlsbergensis and its growth-inhibition by thiamine was investigated. Growth of the yeast was always inhibited by addition of thiamine notwithstanding its inoculum prepared in the medium with or without pyridoxal and thiamine. If thiamine was added to the broth when growth of the yeast reached OD 0.05-0.10,growth-stimulation rather than inhibition was observed. Growth of the yeast, if a heavy inoculum was used, was not retarded by addition of thiamine.
    View full abstract
    Download PDF (533K)
  • STUDIES ON THE ANTIVITAMIN B-6 ACTION ON LACTIC ACID BACTERIA AND YEAST : (VI) GROWTH OF SACCHAROMYCES CARLSBERGENSIS 4228 IN THE MEDIA CONTAINING AN INDIVIDUAL AMINO ACID AS THE SINGLE NITROGEN SOURCE AND ITS GROWTHINHIBITION BY THIAMINE
    Yasuo KAKIUCHI
    Article type: Article
    1969 Volume 40 Issue 6 Pages 459-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_459_2
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Although inhibition of the initial growth of Saccharomyces carlsbergensis by thiamine was observed in the media containing an individual amino acid or (NH_4)_2SO_4 as the single nitrogen source instead of the Atkin's medium, the inhibition in the medium containing alanine, serine, methionine or threonine by thiamine was stronger and more lasting to the later growth phase. In the medium containing phenylalanine, growth-stimulation rather than inhibition by thiamine was observed. In the media containing two amino acids of different types, growth-inhibition by thiamine was shown to be incomplete as compared with that inthe media containing alanine, serine or methionine.
    View full abstract
    Download PDF (175K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 460-461
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_460
    JOURNAL FREE ACCESS
    Download PDF (298K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1969 Volume 40 Issue 6 Pages 461-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_461_1
    JOURNAL FREE ACCESS
    Download PDF (187K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 461-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_461_2
    JOURNAL FREE ACCESS
    Download PDF (187K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 461-462
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_461_3
    JOURNAL FREE ACCESS
    Download PDF (322K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 462-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_462_1
    JOURNAL FREE ACCESS
    Download PDF (188K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 462-464
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_462_2
    JOURNAL FREE ACCESS
    Download PDF (402K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 464-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_464_1
    JOURNAL FREE ACCESS
    Download PDF (186K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1969 Volume 40 Issue 6 Pages 464-465
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_464_2
    JOURNAL FREE ACCESS
    Download PDF (337K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 465-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_465_1
    JOURNAL FREE ACCESS
    Download PDF (202K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 465-466
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_465_2
    JOURNAL FREE ACCESS
    Download PDF (345K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1969 Volume 40 Issue 6 Pages 466-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_466_1
    JOURNAL FREE ACCESS
    Download PDF (195K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 6 Pages 466-
    Published: December 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.6_466_2
    JOURNAL FREE ACCESS
    Download PDF (195K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top