Abstract
Influence of inoculating conditions on growth of Saccharomyces carlsbergensis and its growth-inhibition by thiamine was investigated. Growth of the yeast was always inhibited by addition of thiamine notwithstanding its inoculum prepared in the medium with or without pyridoxal and thiamine. If thiamine was added to the broth when growth of the yeast reached OD 0.05-0.10,growth-stimulation rather than inhibition was observed. Growth of the yeast, if a heavy inoculum was used, was not retarded by addition of thiamine.
