Abstract
The growth of Kloeckera apiculata in the broth containing thiamine and imidazolothiamine (I) or benzylimidazolothiamine (II) was fairly retarded because of the strong inhibition of thiamine uptake by (I) or (II). Both the growth of thiamine-uptaken cells in the broth containing (I) or (II) and that of (I) or (II)-uptaken cells in the broth containing thiamine were not so much retarded because of the high content of thiamine in cells due to the easy uptake of the added thiamine. It was found that the growth after 8 hours was correlated with thiamine content or accumulation ratio of thiamine to antithiamine compound in cells after 2 hours. Coefficient of correlation between the growth and thiamine content or accumulation ratio was about 0.5 or 0.8 as shown in Fig. 5.
