Abstract
It was found by paper chromatography that two new derivatives of pyridoxine were formed in the incubation mixture containing crystalline β-galactosidase from Escherichia coli, o-nitrophenyl-β-D-galactopyranoside and pyridoxine. These derivatives were isolated as powdered preparations and assumed to be pyridoxine 4'-β-galactoside and a small amount of pyridoxine 5'-β-galactoside, respectively, from various data, viz., R_F values, absorption spectra at acidic, neutral and alkaline pH, products by acid hydrolysis and by hydrolysis with glycosidases, behaviour to Gibbs' test and absorption spectra in saturated boric acid solution. It was also observed that β-galactosidase synthesized galactosides of several vitamin B_6 derivatives.
