Abstract
The influence of BHT-hydroperoxide (2,6-di-tert-butyl-4-methyl-4-hydroperoxy-2,5-cyclohexadine-1-one) formed from BHT by heating BHT with potassium hydroxide under the condition used for saponification of vitamin A preparations, ^<1)> on the colorimetric determination of vitamin D by Nield's reagent was examined. It was confirmed that the influence of BHT-hydroperoxide prepared from BHT, ^<8)> on the colorimetric determination of vitamin D was not due to the oxidative decomposition of vitamin D by BHT-hydroperoxide, and that the color reaction of vitamin D with Nield's reagent was inhibited by the presence of BHT-hydroperoxide. Elimination of BHT-hydroperoxide from vitamin D was achieved successfully by gel filtration using Sephadex LH-20 as bed and chloroform as eluting solvent and quantitative recovery of vitamin D was obtained.
