Abstract
Reactions of N-bromosuccinimide (NBS) to ascorbic acid (AsA), other reductones, enzyme protein and amino acids were investigated. NBS reacted with equimolecular amount of AsA, triose reductone or reductic acid, and with the mixture of reductone III and B, without any preferential oxidation of AsA to the other reductones. The titrated values of these reductones by NBS method coincided almost with those by indophenol method. The reaction mixture of 3,4-dihydroxyphenylalanine (DOPA) and phenolase caused some interference to the NBS method. This interference was not due to the reaction of NBS to either DOPA or phenolase. On the other hand, amino acids such as tryptophan, glycine and phenylalanine caused no interference to the NBS method. From these results, the availability of the NBS method for vitamin C assay was discussed.
