Abstract
To clarify the thiamine activity of O-benzoylthiamine and thiamine-thiazolone, O-benzoylthiamine or thiamine-thiazolone were given orally or parenterally to Wistar rats which on the thiamine deficient diet. The results obtained were as follows : (1) In thiamine-thiazolone administered rats by either oral or parenteral administration, growth was depressed markedly. While, In O-benzoylthiamine administered groups, good growth was observed. (2) Thiamine concentration in liver, kidneys, brain and blood decreased significantly in thiamine-thiazolone administered groups. In O-benzoylthiamine administered rats, liver thiamine levels decreased slightly and no significant change was observed in thiamine levels of other tissues. (3) Transketolase activities in liver of thiamine-thiazolone administered rats decreased markedly and that of O-benzoylthiamine administered rats decreased slightly. In vitro addition of thiamine diphosphate to liver homogenate of these rats resulted in a recovery of transketolase activity.
