Abstract
The incorporation of L-dehydroascorbic acid (DAsA) into human erythrocytes in vitro was investigated. DAsA was added to the mixture of 10% of erythrocytes and 90% of isotonic phosphate buffer (pH 7.4) at 37℃. The incorporation was finished within one minute and some of the incorporated DAsA was reduced L-ascorbic acid (AsA) by GSH and other reducing substances in the erythrocytes. The concentration of erythrocyte DAsA was proportional to that of the extracellular DAsA (100〜77% of external concentration) and the production of AsA was dependent on reducing substances in erythrocytes, mainly on GSH. When a small quantity of DAsA (5mg/dl) was added, the reduction of DAsA was due to GSH alone, but when large quantities of DAsA (100 mg/dl) was added, the reduction of DAsA was affected by other reducing substances.
