Abstract
O, S-Dibenzoyl thiamine hydrochloride (DBT-HCl) was examined for the mutagenicity by the dominant lethal test and in vivo cytogenetic test. In the dominant lethal test, (C3H × SWV/V) F_1 male mice were orally given a single dose of 1,000mg/kg or five successive doses of 300mg/kg/day of DBT-HCl. Mean numbers of viable implants per female in the experimental groups did not differ significantly from those in the vehicle (5% gum arabic solution) at any periods examined. In vivo cytogenetic effects of DBT-HCl were examined on bone marrow of CF#1 male mice receiving the similar treatment as in the dominant lethal test. There was no increase of chromosome aberration in any group treated with DBT-HCl as compared with the contro1. These results indicate lack of mutagenicity of DBT-HCl in the present experimental conditions.
