Abstract
Intrinsic factor (IF) in rat gastric mucosal extract was bound efficiently to vitamin B_12-Sepharose without significant changes in its nature to produce IF-vitamin B_12-Sepharose. Purification of intestinal receptor for IF-vitamin B_12 complex was performed by affinity chromatography using the IF-vitamin B_12-Sepharose as an affinity adsorbent with recovery of 48.5% and specific activity increased 335 folds of original sample.
