Abstract
Two method have been developed for the determination of vitamin A in serum and liver by high-speed liquid chromatography (HSLC) using on-line spectrofluorometer. Liver in which vitamin A is present as palmitate, was homogenized and extracted with n-hexane and the n-hexane extract was evaporated under N_2 gas at 30℃. The residue was dissolved in an isopropanol solution of benzo (a) pyrene, and subjected to HSLC using Permaphase ODS column and isopropanol・ethanol・H_2O (56 : 16 : 28) as mobile phase, and vitamin A was determined by internal standard method. Serum in which vitamin A was present as alcohol, was extracted with n-hexane identically. The residue was dissolved in chloroform and subjected to HSLC using JASCOPACK WC-03 column and chloroform・isooctane (0.5 : 99.5) as mobile phase. Vitamin A was determined from calibration curves. The proposed HSLC methods were not disturbed by other fat soluble substances and the detection limits were one tenth as compared with conventional methods.
