Abstract
We have reported the marked increase of AST activity in the liver of the diabetic mice, spontaneously non-obese diabetic (NOD) mice and alloxan-diabetic (Allo) mice, as compared with that of control Institute of Cancer Research (ICR) mice. In this paper we determined vitamin B_6, pyridoxamine (PM) and pyridoxal (PL) in the liver of diabetic NOD and ICR mice by the HPLC method. More B_6 was observed in the diabetic liver than in the control liver, and this change was especially caused by the increase in the PM fraction but not in the PL fraction. On the other hand, abnormally high AST activity was observed in the cytosolic fraction of the liver but not in the mitochondrial fraction. A significant correlation between s-AST and PM was clearly recognized in the diabetic liver against no relationship in the control liver. These findings suggest a role of B_6 in gluconeogenesis of the liver.
