Abstract
The changes of intracellular B_2 and B_6 contents during growth of Euglena gracilis Z and its bleached mutant (strain SM-ZK) were studied. Intracellular B_2, FMN and FAD showed constant levels during growth of the light- and dark-adapted green wild type cells (GL and GD, respectively), but decreased temporarily in the logarithmic growth phase in the light- and dark-adapted bleached cells (BL and BD, respectively). All the Euglena cells contained B_2 and its coenzyme forms at a constant ratio (B_2 : FMN : FAD = 4:25:70) during their growth. On the other hand, the level of coenzyme B_6, PLP, increased in the logarithmic phase and then was kept constant in GL and GD cells. In BL and BD cells; PLP was significantly accumulated in the early logarithmic phase, when the amount of total B_6 was half of that in GL cells grown for 6 days. The maximum content of total B_6 increased about 1.5 times under illumination in both E. gracilis Z and SM-ZK.
