Abstract
In order to simplify the microbiological assay of vitamins (biotin, nicotinamide; NiA-NH_2 and pantothenic acid ; PaA), a modified method using microplate was studied. It was found that the growth responses to biotin, NiA-NH_2 and PaA of Lacto-bacillus plantarum ATCC 8014 were remarkably affected by addition of carbon dioxide (CO_2) to incubation chamber. Under suitable CO_2 condition, the stable and satisfactory results were obtained. When these vitamin contents in a vitamin mixture and human blood were determined by this method, these values were agreed well to those obtained by the conventional microbiological assay method. Concerning with biotin, the correlation coefficient between the values obtained by the two methods was 0.99258. Possibility for practical use of this new microbiological assay method was suggested.
