Abstract
In the in vitro lipid peroxidation dependent on microsomal cytochrome P-450, the production of thiobarbituric acid-reactive substance (TBARS) was increase by the addition of ethanol in the reaction mixture. The increase of TBARS was inhibited by the addition of flavin adenine dinucleotide (FAD), superoxide dismutase or catalase, and was also inhibited by uric acid or mannitol but not inhibited by sodium azide. The reaction of p-nitroso-N, N-dimethylaniline and hydroxyl radical (・OH) was inhibited by FAD, and FAD was decreased by the reaction of ・OH production. These results suggest that the active oxygen radical, such as ・OH, generates by the metabolism of ethanol in microsome reacted more rapidly with FAD than unsaturated fatty acid, and then the lipid peroxidation is inhibited.
