Abstract
Emmerie-Engel's method of colorimetric determination of α-tocopherol was reexamined. The extinction value at 520mμ of colored solution followed Lambert-Beer's law in the limits of 0.01-0.5mg α-tocopherol. The stability of reagents and of colored solution was examined. Oxidized products of α-tocopherol separated by the aluminachromatography, and phenolic antioxidants presented color by addition of reagents as was the case with α-tocopherol. The method of removing them was described.
