Abstract
It has been reported that embryo of cereals, especially of rice, wheat and barley are suitable raw materials for the production of riboflavin by Eremothecium ashbyii. It is, however, necessary to remove the excess of inorganic substances in the embryo by steeping in water, since the embryo contains so much inorganic substances as they inhibit the growth of the fungus owing to the high osmotic pressure. But, by this treatment the nutrient substances in embryo are also lost. An attempt was therefore made to omit the steeping process by creating a new strain of the fungi, tolerable of high osmotic pressure. This was succeeded by cultivation on embryo extract agar supplemented with 5% salt. It was however verified that the new strain had a lower ability of synthesizing riboflavin.
