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Chikataro KAWASAKI
Article type: Article
1955 Volume 9 Pages
1-13
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
13-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
13-
Published: 1955
Released on J-STAGE: December 22, 2017
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Tadashi KAWAKAMI
Article type: Article
1955 Volume 9 Pages
14-18
Published: 1955
Released on J-STAGE: December 22, 2017
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Thiaminase I, obtained from different sources, was treated with H_2S, and under conditions where no decomposition of thiaminase was recongnizable, their base exchange reactions were observed. On the basis of stability, thiaminases were classified into the stable and unstable ones. The latter was again divided into two groups, those unstable both in aniline and pyridine base exchange reaction, and those unstable only in aniline exchange reaction, but stable in pyridine exchange reaction, or vice versa. A method for purifying thiaminase of Bacillus tkiaminolyticus with acetone in combination with H_2S was described.
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
18-
Published: 1955
Released on J-STAGE: December 22, 2017
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Goichiro KATSUI, Michiko IIZIMA
Article type: Article
1955 Volume 9 Pages
19-20
Published: 1955
Released on J-STAGE: December 22, 2017
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Tests of antioxidant activity for vitamin A of quercetin prepared by the treatment with sodium bisulfite of dihydroquercetin isolated from the bark of Douglas fir, resulted in the reconfirmation that antioxidant activity of the substance examined was too weak to be of practical use.
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
20-
Published: 1955
Released on J-STAGE: December 22, 2017
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Goichiro KATSUI, Kimishige ONOZATO
Article type: Article
1955 Volume 9 Pages
21-23
Published: 1955
Released on J-STAGE: December 22, 2017
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Investigations from various viewpoints on the influence of dehydroacetic acid on the oxidation of vitamin A showed that the substance had little or no effect on the prevention of the vitamin from oxidation. Dehydroacetic acid might be used as a antifungal agent for foods and pharmaceuticals containing vitamin A, since the substance does not promote the oxidation of vitamin A.
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Koji KIMURA
Article type: Article
1955 Volume 9 Pages
23-25
Published: 1955
Released on J-STAGE: December 22, 2017
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The fluorometric method of Ogawa for vitamin C was compared with the colorimetric method of Roe in the modification of Teruuchi et al. using 13 varieties of vegetable tissues as well as 16 varieties of animal ones. The vitamin C values found with vegetable tissues were similar, but those for animal tissues differed markedly between the two methods. In the latter, the fluorometric values was much higher than the colorimetric ones. The specificity of the fluorometric method may therefore be considered to be inferior to the colorimetric, at least with animal tissues.
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Tetsuzo TAKAHASHI
Article type: Article
1955 Volume 9 Pages
26-28
Published: 1955
Released on J-STAGE: December 22, 2017
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The method was improved so as to be capable of determining vitamin C in a small amount of blood. The total vitamin C in the blood was determined by this method on 60 adults. The mean level was 0.64±0.25mg%.
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
28-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
28-
Published: 1955
Released on J-STAGE: December 22, 2017
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Tatsuo MATSUDA, Fumio GOTO
Article type: Article
1955 Volume 9 Pages
29-32
Published: 1955
Released on J-STAGE: December 22, 2017
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A solvent extraction procedure with phenol-butanol for purifying vitamin B_<12> was studied. The extraction of vitamin B_<12> and B_<12>, from water solution increased with increase in the concentration of phenol in the solvent Change in pH had almost no effect on the extraction of vitamin B_<12>, while that of vitamin B_<12> grew lower with decrease of pH. Vitamin B_<12> could be recovered from the solvent phase by extracting with water and ether, benzene, or carbontetracholoride. The mixed solvents of phenol 40-60 g per 100 ml of butanol were capable to extract vitamin B_<12> in satisfactory yields with a high selectivity.
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Tatsuo MATSUDA
Article type: Article
1955 Volume 9 Pages
32-37
Published: 1955
Released on J-STAGE: December 22, 2017
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No marked difference in adsorption of vitamin B_<12> was found between Amberlite IR C-50 and carbon impregnated IRC-50. The former resin was used to purify vitamin B_<12>. The optimum pH for adsorption ranged from 1.5 to 3.0 (ordinarily 2.5), 0.1 N HCl solution in 75% acetone or 50% isopropyl alcohol was used as an eluting agent. When sample solution of vitamin B_<12> contained much impurities, adsorption of the vitamin was greatly obstructed and for elution a large amount of solvent was necessary. With partially purified sample solution, adsorption took place quickly and less amount of solvent was repuired, but purification was not improved thereby. In the latter case, however, chromatographic seperation succeeded more satisfactorily.
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Tatsuo MATSUDA, Keichi NUNOME
Article type: Article
1955 Volume 9 Pages
37-39
Published: 1955
Released on J-STAGE: December 22, 2017
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Synthesis of vitamin B_<12> by Streptomyces hachijoensis, capable of producing a new antibiotic trichomycin, was studied. Addition of CoCl_2 to the culture medium increased the synthesizing capacity by 3.0-3.2 times, and 29-32μg/100 cc (Lactobacillus leickmannii value) was obtained by the shaking flask culture and 20-23μg/100 cc by the tank culture. Trichomycin and vitamin B_<12> are contained in mycelium, but during the extraction of trichomycin, the vitamin passes into waste liquor so that the latter may be used as a source material for isolation of the vitamin.
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Tatsuo MATSUDA
Article type: Article
1955 Volume 9 Pages
40-44
Published: 1955
Released on J-STAGE: December 22, 2017
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The isolation of vitamin B_<12> from trichomycin fermentations was studied. It was first adsorbed by charcoal, followed by elution, extraction with phenol-butanol, and chromatographic separation on Amberlite IRC-50 with 0.1N hydrochloric acid in 75% acetone. The eluate was passed through a column of Amberlite IR-4B to remove hydrochloric acid and finally the vitamin was crystallized as red needles from aqueous acetone. The counter-current distribution preocedure was applied to the concentrated eluate of charcoal adsorbate for the determination of B_<12>, B_<12>b and B_<12>-like substances. The crystals obtained by two procedures, with and without KCN treatment, were proved to be vitamin B_<12> by their characteristic absorptions in ultraviolet and visible spectra and countercurrent distribution curve.
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Sachiko UENO
Article type: Article
1955 Volume 9 Pages
45-47
Published: 1955
Released on J-STAGE: December 22, 2017
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Investigations on the vitamin B_<12> content of some species of fishes and weeds in Lake Biwa conducted in order to examine their nutritional and feed supplement values showed that 1-2μg% of the vitamin was contained in flesh and 10-47μg% in internal organs of the fishes tested, and 5-20μg% of the vitamin was found in the several kinds of shell fishes examined, while some of the fresh-water weeds contained as much vitamin B_<12> as 16μg% for dry basis.
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Fujiya AOKI
Article type: Article
1955 Volume 9 Pages
48-52
Published: 1955
Released on J-STAGE: December 22, 2017
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One of the 96 yeast-like strains, isolated from human mouths has been found to have a thiaminase action. The enzyme produced by this strain has proved to be "thiaminase II" by the precise biological investigations.
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Fujiya AOKI
Article type: Article
1955 Volume 9 Pages
53-57
Published: 1955
Released on J-STAGE: December 22, 2017
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Mycological investigation on the strain, having thiaminase action, demonstrated that it belonged to Trichosporum among Fungi imperfecti. The decision whether this strain is a new species or not, however, needs further investigations.
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Tutou AIHARA, Kazuo SATO
Article type: Article
1955 Volume 9 Pages
58-60
Published: 1955
Released on J-STAGE: December 22, 2017
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Colorimetric determination of folic acid presented in the U.S.P. can not be applied for the vitamin in multivitamin preparations owing to the inhibition of other vitamins, especially riboflavin and ascorbic acid, contained in the preparations. The present paper reports a modified colorimetric determination of folic acid achieved by the elimination of inhibiting substances based on the difference of adsorbability of acid clay depending on pH value.
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
60-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
60-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
60-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
61-67
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
67-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
67-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
68-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
68-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
68-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
69-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
69-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
69-70
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
70-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
70-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
71-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
71-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
71-72
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
72-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
72-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
72-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
72-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
72-73
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
73-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
73-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
73-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
73-74
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
74-
Published: 1955
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
74-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1955 Volume 9 Pages
74-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1955 Volume 9 Pages
75-
Published: 1955
Released on J-STAGE: December 22, 2017
JOURNAL
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