ウコン中のCurcuminの高速液体クロマトグラフィーによる定量

浅川 直樹; 津野 昌紀; 服部 禎一; 上山 政晤; 篠田 愛信; 三宅 康夫; 影井 健吾

doi:10.1248/yakushi1947.101.4_374

Determination of Curcumin Content of Turmeric by High Performance Liquid Chromatography

NAOKI ASAKAWA, MASANORI TSUNO, TEIICHI HATTORI, MASAAKI UEYAMA, AISHIN SHINODA, YASUO MIYAKE, KENGO KAGEI

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Keywords: HPLC, determination, curcumin, Curcuma longa, Nucleosil C₁₈

JOURNAL FREE ACCESS

1981 Volume 101 Issue 4 Pages 374-377

DOI https://doi.org/10.1248/yakushi1947.101.4_374

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Abstract

A method of quantitative determination of curcumin in Curcuma longa was established by using high performance liquid chromatography. Curcumin contents of methanol, aqueous methanol-(4 : 6) and water-extracts of turmeric (rhizomes of Curcuma longa L.) were determined by high performance liquid chromatography (HPLC) by use of Nucleosil C₁₈ column as a stationary phase, a mixture of CH₃CN : H₂O : CH₃COOH (51 : 49 : 5) as a mobile phase and benzyl benzoate as an internal standard. The present method is preferable to other analytical methods with regard to accuracy, simplicity and speed.

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