A Simple and Rapid Method of Radioimmunoassay of Cyclic AMP with the Cell Harvester

Abstract

The characteristics of the cell harvester (LM-101, Labo Mash, Labo Science Co.) were developed for separation of bound and free labeled antigens or haptens (B/F separation) in radioimmunoassay to determine cyclic AMP. Sensitivity and accuracy of the Labo Mash filtration method were similar to those of the Millipore filtration method or the double antibody method. The filtration method by use of the cell harvester is effective for B/F separation in radioimmunoassay, since this procedure is very quick and simple.