Colorimetric and Fluorometric Determination of Cartap by Use of Ion Association with Calcon

Abstract

Cartap could be determined colorimetrically and fluorometrically by use of ion pair extraction with calcon, which was used for fluorometric reagent of aluminium ion. Cartap (S, S'-[2-(dimethylamino) trimethylene]-bis (thiocarbamate)) was hydrolyzed to nereistoxin, and then 0.5 ml of 0.2% calcon and 4 ml of dichloromethane was added to 1 ml of the nereistoxin solution in acidic medium. The mixture was shaked mechanically for 1 min, followed by separation and centrifugation of the extracted solution. The solution showed max. absorbance at 530 nm, and that obeyed Beer's law in the range of 1 to 20μg/ml. Then, the solution was made to be fluorescence one by the addition of aluminium ion in acidic medium. Samples including lower content of cartap could be determined by the measurement of fluorescence intensity.