Discrimination of the Aglycones of Diosgenin, Pennogenin Glycosides and Their Corresponding Proto-Type Glycosides by the Electron Impacting Mass Spectrometry

Abstract

By direct measurement of electron impacting mass spectrometry of naturally occurring diosgenin, pennogenin glycosides and their corresponding furostanol glycosides, without leading to the volatile derivatives, the information with regards to the genuine aglycones of the respective glycosides could be obtained. Namely, diosgenin, pennogenin glycosides and their respective furostanol glycosides exhibited characteristic peaks at (m/z 139), (m/z 155, 153, 127, 126), (m/z 576, 139, 126), (m/z 574, 127, 126), respectively, being distinguishable easily from each other.