Anthraquinones Production by Hairy Root Culture in Cassia obtusifolia

Abstract

Hairy roots were induced in seedlings of Cassia obtusifolia inoculated with Agrobacterium rhizogenes. Established hairy root clone in shaking culture synthesized several anthraquinones; 8-O-methylchrysophanol, chrysophanol, emodin and physcion. Their yields were maximized in the late exponential growth phase of shaking-culture. The anthraquinones composition of hairy roots was different from that of mature seeds (Ketsumeishi) and relatively similar to that of the primary roots of seedling.