Determination of Sennoside A in Oriental Pharmaceutical Decoctions Containing Rhei Rhizoma by Ion-Pair High-Performance Liquid Chromatography

Abstract

A simple method using ion-pair high-rerformance liquid chromatography was established for the rapid and precise determination of sennoside A in oriental pharmaceutical decoctions containing Rhei Rhizoma. This method was compared with other two methods, i.e. ion-suppression and phosphate buffer methods. Sennoside A was eluted without interference in this ion-pair method, while the determination of sennoside A was interfered by co-existing components in the other two methods.