A Simultaneous Determination of Honokiol and Mangolol in Oriental Pharmaceutical Decoctions Containing Magnolia Bark by Ion-Pair High-Performance Liquid Chromatography. II

Abstract

A simple method using ion-pair high-performance liquid chromatography was established for the rapid and precise determination of honokiol (3', 5-di-2-propenyl-1, 1'-biphenyl-2, 4'-diol) and magnolol (5, 5'-di-2-propenyl-1, 1'-bi-phenyl-2, 2'-diol) in eighteen species of oriental pharmaceutical decoctions containing Magnolia bark. An ODS column and a mixed solvent system of water involving 10 mM tetra-n-amylammonium bromide (TAA) and acetonitorile (4 : 6) as a mobil phase were used for the separation. Honokiol and magnolol were eluted without interference of other coexisting components within 12 min.