Recent Progress in Detection-Oriented Derivatization

Abstract

Derivatization reactions to enhance the sensitivity and selectivity of gas and high-performance liquid chromatographic detection of biologically important substances are reviewed, with emphasis on the papers reported in the last decade. The achiral and chiral reagents for various functional groups, such as amino, carboxy, thiol, carbonyl and hydroxy groups, include phthalimidyl, quinolyl, quinoxalyl, benzoxazolyl and benzoxadiazolyl analogous having a reacting group to make a covalent bond with analytes. The derivatization methods for increasing sensitivity in liquid chromatography combined with mass spectrometry are also discussed.