On the Biological Assay of Japanese Senega

Abstract

Existing literature on the hemolytic methods for potency determination of saponinbearing drugs was examined. In the activity determination of Japanese senega, hemolytic index was obtained by using rabbit blood in a phosphate buffer solution of pH 7.4, Merck's pure Saponin as the standard. As a result, it was found that the domestic product possessed a potency equal to the drug of S. B. Penick & Co., which is assumed to be the Northern senega (cf. Table I)