Studies on Removal of Pyrogen. I

Removal of Pyrogen with Ion Exchange Resin

Abstract

Water of pH 9, giving positive tetrabromophenolphthalein (TBP) reaction, was passed through a column (1×10cm.) filled with 10g. of ion exchangers, Cl-type Amberlite IRA-400, H-type Amberlite IR-120, or Na-type Amberlite IR-120, at the rate of 2cc./minute. The effluent water was found to give negative pyrogen test and weak TBP reaction. The same adsorption treatment of pyrogenpositive serum through a column of Na-type Amberlite IR-120 also resulted in the removal of pyrogen from the serum with only a small loss of antibody value.