Preparation of Succinoxidase from E. coli irradiated with Supersonic Wave

Abstract

Colt bacilli were destroyed by irradiation of supersonic waves and succinic oxidase was extracted. It was found that irradiation of 560Kc., 1800V., and 0.29-0.30A, for 15 minutes resulted in almost complete extraction of succinic oxidase in cellfree state. When the coli bacilli are destroyed under above conditions and centrifuged at 10, 000r.p.m., succinic oxidase activity is detected in the microsomes of 20-50mμ. The succinic oxidase so extracted is precipitated in the majority by 60-70% saturation of ammonium sulfate.