Studies on Fluorometric Analysis of Drugs. I

Fluorometry of Powdered Drugs

Abstract

It was found that organic compounds in powder form emit fluorescence of various intensities and color tones by the irradiation of ultraviolet light of a definite wave length. Finding that such fluorescence was related to chemical structure of each substance, measurement of the intensity and wave length of the fluorescence was thought to be capable of being an aid for the identification of a substance, discrimination of purity, and the study of chemical structure of organic compounds, and this method was applied to the drugs adopted in formularies, dyes, antibiotics, and crude drugs.
As a first step, attempt was made to measure the intensity of fluorescence by the degree of blackening of photographic film and examinations were made on the apparatus of measurement, blackening of sensitizing film, its reproducibility and lineality, and the method of indicating the fluorescence intensity.